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Related Experiment Video

Updated: Apr 15, 2026

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Live-Cell STED Microscopy with Genetically Encoded Biosensor.

Natalia M Mishina^1,2, Alexander S Mishin^1,2, Yury Belyaev³

¹†Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.

|April 15, 2015

Summary

This summary is machine-generated.

This study demonstrates live-cell super-resolution microscopy using a hydrogen peroxide (H2O2) biosensor. The technique allows for enhanced imaging of cellular processes and H2O2 production in vivo.

Keywords:

HyPer STED biosensor hydrogen peroxide microscopy super-resolution imaging

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Stimulated emission depletion (STED) microscopy offers high spatial and temporal resolution for live-cell imaging beyond the diffraction limit.
Current super-resolution methods primarily use fluorophore positions, limiting broader applications.
Expanding super-resolution techniques requires novel approaches for dynamic biological processes.

Purpose of the Study:

To apply live-cell STED microscopy to a dynamic biosensor for enhanced cellular imaging.
To investigate the utility of the fluorescent H2O2 sensor HyPer2 for subdiffraction imaging.
To demonstrate the potential of fluorescent protein-based biosensors in super-resolution experiments.

Main Methods:

Live-cell STED microscopy was performed using the HyPer2 fluorescent biosensor.
Subdiffraction imaging was achieved by localizing fluorescent emission.
Cellular hydrogen peroxide (H2O2) production was monitored by tracking probe brightness.

Main Results:

Filamentous structures were imaged with superior resolution using STED microscopy.
The dynamic production of H2O2 within living cells was successfully traced.
The HyPer2 sensor enabled super-resolution imaging of H2O2 dynamics.

Conclusions:

Live-cell STED microscopy of the HyPer2 sensor is feasible and provides enhanced resolution.
Fluorescent protein-based biosensors show significant potential for in situ and in vivo super-resolution studies.
This approach expands the application of super-resolution microscopy to dynamic biochemical processes.