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[Peculiarities of Proteus mirabilis extracellular metalloproteinase biosynthesis]

N M Zamaliutdinova, M R Sharipova, L M Bogomol'naia

    Izvestiia Akademii Nauk. Seriia Biologicheskaia
    |April 16, 2015
    PubMed

    Insights

    Proteus mirabilis bacteria secrete metalloproteinase, but glucose suppresses its production. Urea and natural urine enhance metalloproteinase (proteinase) biosynthesis by this bacteria strain.

    Area of Science:

    • Microbiology
    • Enzymology
    • Bacterial Physiology

    Background:

    • Proteus mirabilis is known to produce various enzymes, including metalloproteinases.
    • Understanding the regulation of metalloproteinase biosynthesis is crucial for potential biotechnological applications.

    Purpose of the Study:

    • To investigate the biosynthesis of metalloproteinase by Proteus mirabilis 5127-1.
    • To determine the influence of glucose and urea on metalloproteinase production.
    • To identify optimal conditions for metalloproteinase secretion.

    Main Methods:

    • Culturing Proteus mirabilis 5127-1 on various media (synthetic, LB glucose, artificial urine, natural urine).
    • Analyzing bacterial supernatant for metalloproteinase activity and isoforms via SDS-PAGE.
    • Quantifying culture productivity in relation to proteinase synthesis.

    Main Results:

    • Proteus mirabilis 5127-1 secretes metalloproteinase in two isoforms (52 and 50 kDa).
    • Proteinase synthesis was completely inhibited by synthetic media and LB glucose.
    • Urea addition significantly increased proteinase synthesis, with maximal productivity observed in natural urine.
    • Proteinase production in artificial urine was delayed and appeared as a single isoform.

    Conclusions:

    • Glucose and synthetic media suppress metalloproteinase production in P. mirabilis.
    • Urea and natural urine are effective inducers of metalloproteinase biosynthesis.
    • The composition of the growth medium critically affects the yield and isoforms of metalloproteinase secreted by P. mirabilis.

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