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Published on: December 13, 2012
B-ALL minimal residual disease flow cytometry: an application of a novel method for optimization of a single-tube
Aaron C Shaver1, Bruce W Greig2, Claudio A Mosse3
1From the Vanderbilt University Medical Center, Nashville, TN, and aaron.shaver@vanderbilt.edu.
Objectives:
Optimizing a clinical flow cytometry panel can be a subjective process dependent on experience. We develop a quantitative method to make this process more rigorous and apply it to B lymphoblastic leukemia/lymphoma (B-ALL) minimal residual disease (MRD) testing.
Methods:
We retrospectively analyzed our existing three-tube, seven-color B-ALL MRD panel and used our novel method to develop an optimized one-tube, eight-color panel, which was tested prospectively.
Results:
The optimized one-tube, eight-color panel resulted in greater efficiency of time and resources with no loss in diagnostic power.
Conclusions:
Constructing a flow cytometry panel using a rigorous, objective, quantitative method permits optimization and avoids problems of interdependence and redundancy in a large, multiantigen panel.
