Neutrophil CD64 expression - comparison of two different flow cytometry protocols on EPICs MCL and the Leuko64(™)
Oskar Eriksson1, Lena Douhan Håkansson, Malgorzata Karawajczyk
1Division of Clinical Chemistry and Pharmacology, Department of Medical Sciences, Uppsala University , Uppsala , Sweden.
Objective:
To evaluate the Trillium Diagnostics Leuko64(™) assay on Abbott Celldyn Sapphire haematology analyser compared to two flow cytometry protocols on Beckman Coulter EPICS MCL flow cytometer.
Materials And Methods:
CD64 expression on neutrophils was determined by two flow cytometry protocols and by a commercial assay on an automatic haematology analyser. The inclusion of study subjects was based on elevated procalcitonin (PCT) values, identifying patients where a systemic infection was suspected. Healthy blood donors were used as a reference group.
Results:
Statistically significant correlations between the Trillium Diagnostics Leuko64(™) assay and the flow cytometry methods were found when measuring neutrophil CD64 expression.
Conclusions:
The good correlation between a reference method and an automated haematology analyser method for CD64 expression on neutrophils supports introduction of the latter assay for routine use as an independent biomarker of bacterial infection and inflammation.
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