Related Experiment Video
Updated: Apr 14, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
[Isolation and identification of bacteria with ferro-oxidase activity]
Objective:
We screened and isolated Ferro-oxidase producing bacteria, for adsorbing iron and manganese.
Method:
The strains producing Ferro-oxidase were isolated from three samples of water. Ferro-oxidase producing strains were screened in shake flask culture, and identified according to morphological features, physiological and biochemical analysis as well as 16S rRNA gene sequence analysis.
Results:
We isolated a bacterium S9. The strain was identified as Sphaerotilus natans. This strain had strongest adsorption on iron and manganese among the strains we identified, with 29.02 mg/g iron adsorption amount in water, and 66.77% adsorption rate for 4 hours' adsorption. When the adsorption time is 6 h, the adsorption amount of manganese was 34.49 mg/g, and the adsorption rate was 70.68%. The optimum temperature and pH value of Ferro-oxidase were 30 degrees C and 7.5, respectively. Mg2+, Na+, K+ could activate Ferro-oxidase, whereas Cu2+ had little impact. While Mn2+, Zn2+ could strongly inhibit Ferro-Oxidase, Pb2+, Ag+ had only modest inhibitory effect.
Conclusion:
Strain S9 had a high Ferro-oxidase activity, and has application potential in sewage treatment.
More Related Videos
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
05:57Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment
Published on: November 15, 2012
Related Concept Videos
Differential Staining Technique
Special Staining Techniques
Techniques for Isolation of Pure Cultures
Methods of Classification and Identification
Microbes and Other Elemental Cycles