Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 14, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

8.1K

Carbon nanoelectrodes for single-cell probing.

Sean E Anderson1, Haim H Bau

  • 1University of Pennsylvania Department of Mechanical Engineering and Applied Mechanics Philadelphia, PA 19104, USA.

Nanotechnology
|April 17, 2015
PubMed
Summary

Fabricated carbon nanoelectrodes provide a direct conductive path for cell penetration measurements. Electrode impedance variations accurately reflect cell and nucleus entry, validated by circuit models.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An inexpensive, portable, refrigeration-free, ready-to-use microfluidic device for real-time multiplexed molecular detection of HIV, HBV, and HCV.

Biomedical microdevices·2026
Same author

GravSorter: a forward-genetics tool for studying gravity response in <i>Caenorhabditis elegans</i>.

The Analyst·2026
Same author

Smartphone-Linked and Electricity-Free Platforms for Rapid Colorimetric Molecular Detection of Poultry Respiratory Viruses at the Point of Need.

Biosensors·2025
Same author

A self-heating, multi-channel slider cassette for innovative point-of-care molecular diagnostics from whole blood samples.

Biosensors & bioelectronics·2025
Same author

Polymeric microbead arrays for microfluidic applications.

Journal of micromechanics and microengineering : structures, devices, and systems·2025
Same author

Self-actuated microfluidic chiplet for two-stage multiplex nucleic acid amplification assay.

Lab on a chip·2024

Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Development of nanoscale tools for biological applications is crucial.
  • Existing nanoelectrodes often require complex assembly.
  • Need for integrated, easy-to-use nanoelectrodes for cellular studies.

Purpose of the Study:

  • To fabricate and characterize novel carbon nanoelectrodes.
  • To assess the utility of these nanoelectrodes for intracellular measurements.
  • To correlate electrode impedance with cellular penetration events.

Main Methods:

  • Fabrication of carbon nanoelectrodes via pyrolitic deposition within glass pipettes.
  • Etching of pipette tips to expose carbon nanoelectrode tips.
  • In situ measurement of electrode impedance during cell and nucleus penetration.
  • Comparison of experimental data with theoretical circuit models.

Main Results:

  • Successfully fabricated carbon nanoelectrodes with integrated conductivity.
  • Measured distinct impedance changes upon cell and nucleus penetration.
  • Demonstrated a correlation between electrode impedance and penetration depth.
  • Experimental results showed good agreement with theoretical predictions.

Conclusions:

  • The developed carbon nanoelectrodes offer a seamless solution for nanoscale biological measurements.
  • Electrode impedance is a sensitive indicator of cellular and nuclear penetration.
  • This technology facilitates direct nanoscale probing of cellular structures.

More Related Videos

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection
07:34

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Published on: May 13, 2019

10.3K
Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording
07:50

Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording

Published on: October 29, 2021

3.5K

Related Experiment Videos

Last Updated: Apr 14, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

8.1K
Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection
07:34

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Published on: May 13, 2019

10.3K
Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording
07:50

Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording

Published on: October 29, 2021

3.5K