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Updated: May 7, 2026
DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
MIRA-seq for DNA methylation analysis of CpG islands
Marc Jung1, Swati Kadam1, Wenying Xiong1
1Beckman Research Institute, City of Hope, Duarte, CA 91010, USA.
Aim:
To develop a reliable method for whole genome analysis of DNA methylation.
Materials & Methods:
Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provide a detailed description of MIRA and combine it with next generation sequencing platforms (MIRA-seq).
Results:
We assessed the performance of MIRA-seq and compared the data with whole genome bisulfite sequencing.
Conclusion:
MIRA-seq is a reliable, genome-scale DNA methylation analysis platform for scoring DNA methylation differences at CpG-rich genomic regions. The method is not limited by primer or probe design and is cost effective.
