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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Apr 14, 2026

Hybrid µCT-FMT imaging and image analysis

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

A modularly designed fluorescence molecular tomography system for multi-modality imaging.

Guohe Wang¹, Bin Zhang², Yichen Ding¹

¹Department of Biomedical Engineering, Peking University, Beijing, China.

Journal of X-Ray Science and Technology

|April 18, 2015

Summary

This summary is machine-generated.

A new modular fluorescence molecular tomography (FMT) system offers flexible multi-modality imaging for biological research. This adaptable system integrates with X-CT, SPECT, and PET, showing great potential for small animal disease models.

Keywords:

Fluorescence molecular tomography fluorescence imaging multi-modality imaging

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Last Updated: Apr 14, 2026

Hybrid µCT-FMT imaging and image analysis

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Published on: June 4, 2015

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Published on: October 28, 2018

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Published on: June 6, 2025

Area of Science:

Biomedical Engineering
Molecular Imaging
Preclinical Research

Background:

Multi-modality imaging provides complementary data in biological research.
Existing systems may lack flexibility for integration with diverse imaging techniques.

Purpose of the Study:

To develop a modular fluorescence molecular tomography (FMT) system.
To enable seamless integration of FMT with other imaging modalities like X-CT, SPECT, and PET.
To facilitate sequential multi-modality imaging for enhanced biological studies.

Main Methods:

A modularly designed FMT system with rotating CCD camera and excitation light source.
Stationary animal on a horizontally moveable transparent holder in a prone position.
Automatic co-registration of FMT with other imaging modalities.
Phantom and animal experiments to validate system performance.

Main Results:

The developed FMT system functions as a standalone device and integrates with X-CT, SPECT, and PET.
Sequential multi-modality imaging was successfully performed.
Accurate results were obtained from phantom and animal studies.
The system demonstrated flexibility and robust performance.

Conclusions:

The innovative, flexible FMT system is a powerful tool for biological research.
It has significant potential for studying small animal disease models.
The modular design enhances its applicability in multi-modality imaging research.