[Kinetic characteristics of the pyruvate transport system in the wheat chloroplast envelope]

Kinetics of pyruvate transport through the envelope chloroplast vesicles has been studied. For wheat cultivar Bezostaya-1 Km is 26 mM, Vmax--33 Mmol/l h per 1 mg of protein, Hill's coefficient--0.56 and the coefficient of temperature inactivation at 40 degrees C is 0.045 min-1. 7 mM Mg or Ca ions for optimal activity of the system are needed. Chloroplast treatment with trypsin increases the pyruvate transport intensity. The simple analytical method is suggested for pyruvate determination at the concentration of 10-150 micrograms/ml.