Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026
Same author

Divergent toxicity mechanisms of amyloid-beta aggregates arising from a single aggregation reaction.

Cell reports·2026
Same author

PD-1 signaling and PD-1 blockade-mediated tumor control are established at microvillar T cell contacts.

Science immunology·2026
Same author

Single-molecule detection methods to study alpha-synuclein aggregation in postmortem Parkinson's disease brains.

Cell reports methods·2026
Same author

A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Cortisol-resistant CAR-NK cells overcome steroid-induced immunosuppression in lung cancer.

Signal transduction and targeted therapy·2026
Same journal

Modeling and analysis of forward and inverse kinematics for a flexible Stewart platform.

PloS one·2026
Same journal

Barriers and facilitators to healthcare utilization amongst people living with sickle cell disease in the United States: A scoping review.

PloS one·2026
Same journal

Enhancing data completeness in time series: Imputation strategies for missing data using significant periodically correlated components.

PloS one·2026
Same journal

Key targets and mechanisms by which gut microbiota-derived metabolites regulate Alzheimer's disease through the immune - inflammatory pathway: Based on network pharmacology and molecular docking.

PloS one·2026
Same journal

Grid-tied Transformer-less Boost Switched Capacitor Topology (TLBSCT) for PV applications.

PloS one·2026
Same journal

The load-velocity profiles and exercise-specific velocity zones for seven commonly used weightlifting exercises.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Apr 14, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

702

Virtual-'light-sheet' single-molecule localisation microscopy enables quantitative optical sectioning for

Matthieu Palayret1, Helen Armes2, Srinjan Basu3

  • 1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom.

Plos One
|April 18, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a new 3D sectioning method for single-molecule super-resolution microscopy. The technique enhances image contrast and feature visibility by filtering out-of-focus signals using a virtual light-sheet.

More Related Videos

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.8K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.5K

Related Experiment Videos

Last Updated: Apr 14, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

702
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.8K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.5K

Area of Science:

  • Biophysics
  • Microscopy
  • Cell Biology

Background:

  • Single-molecule super-resolution microscopy achieves sub-diffraction limit precision for live-cell protein imaging.
  • Conventional methods often struggle with out-of-focus fluorescence, reducing image quality and quantitative accuracy.

Purpose of the Study:

  • To develop and demonstrate a 3D optical sectioning method for single-molecule super-resolution microscopy.
  • To improve image contrast and reveal fine cellular structures by rejecting out-of-focus signals.

Main Methods:

  • Utilizing discarded fitting information from fluorophore localization to define a virtual light-sheet.
  • Implementing an open-source ImageJ plug-in for easy calibration and analysis of the virtual light-sheet.
  • Applying width and amplitude thresholds to diffraction-limited spots for optical sectioning.

Main Results:

  • Demonstrated effective 3D sectioning in super-resolution microscopy.
  • Achieved qualitative and quantitative imaging improvements by rejecting out-of-focus fluorophores.
  • Enhanced contrast and revealed local features in super-resolution images.

Conclusions:

  • The virtual light-sheet method significantly improves super-resolution image quality.
  • The open-source plug-in offers a versatile tool for existing 2D super-resolution instruments.
  • This technique increases the probability of detecting and quantitatively evaluating emitting fluorophores near the focal plane.