Related Experiment Video

Updated: Apr 14, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.9K

Unusual rotavirus genotypes among children with acute diarrhea in Saudi Arabia

Mahmoud Aly1,2, Aisha Al Khairy3, Sameera Al Johani4,5

  • 1King Abdullah International Medical Research Centre, P.O. Box 22490, Riyadh, 11426, Kingdom of Saudi Arabia. alyma@ngha.med.sa.

BMC Infectious Diseases
|April 18, 2015
PubMed

Insights

Human rotavirus A (RV-A) causes severe diarrhea in infants. This study identified common G and P genotypes in Saudi Arabia, finding G1P[8] as dominant and noting uncommon strains similar to those in other Asian countries.

Area of Science:

  • Virology
  • Pediatric Infectious Diseases
  • Molecular Epidemiology

Background:

  • Human rotavirus A (RV-A) is a primary cause of viral gastroenteritis in infants globally.
  • Understanding circulating RV-A genotypes is crucial for public health surveillance and vaccine efficacy.
  • This study focused on characterizing RV-A genotypes in pediatric diarrhea cases in Riyadh, Saudi Arabia.

Purpose of the Study:

  • To determine the G and P genotypes of human rotavirus A (RV-A) in clinical isolates from children with acute diarrhea.
  • To analyze the prevalence and distribution of these genotypes in Saudi Arabia.

Main Methods:

  • Fecal samples from 541 pediatric patients with acute diarrhea were collected between 2011 and 2012.
  • Rotavirus RNA was extracted using a commercial kit, followed by RT-PCR and sequencing for genotyping.
  • Phylogenetic analysis using the Maximum Likelihood method was employed to study genotype clustering.

Main Results:

  • A total of 171 out of 541 (31.6%) samples tested positive for human RV-A, predominantly in children under 2 years old.
  • The most prevalent P genotype was P[8] (96.7%), with G1P[8] being the dominant strain (61.9%), followed by G9P[8] (16.8%).
  • Uncommon genotypes G1P[4] and G12P[6] were detected and clustered with isolates from other Asian countries, suggesting potential international transmission.

Conclusions:

  • The study identified dominant and uncommon human rotavirus A (RV-A) genotypes in Saudi Arabia, with G1P[8] being the most frequent.
  • The detection of uncommon strains linked to other Asian countries highlights the need for ongoing molecular surveillance.
  • Further research into emerging genotypes and rotavirus seasonality is recommended, particularly post-vaccination introduction in routine childhood immunizations.
Abstract

More Related Videos

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

9.5K
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

9.6K

Related Concept Videos

Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
62
Cholera01:25

Cholera

Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...
91
Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents01:18

Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents

Acute diarrhea, a common gastrointestinal disturbance, is characterized by the rapid evacuation of fluid stools, leading to an excessive weight in fluid. This condition typically arises from disorders affecting intestinal water and electrolyte transport. It can be triggered by an increased osmotic load within the intestine, excessive secretion of electrolytes and water, mucosal exudation of protein and fluid, or altered intestinal motility. The primary risks of acute diarrhea are dehydration...
561
Viruses of Archaea01:29

Viruses of Archaea

Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
669