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Updated: Apr 14, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
Gernot Schmoock1, Mandy Elschner2,3, Lisa D Sprague4
1Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany. gernot.schmoock@fli.bund.de.
Background:
A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers.
Findings:
Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively.
Conclusions:
This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis.
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