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Updated: Apr 14, 2026
Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
Published on: September 5, 2013
Development of a viability standard curve for microencapsulated probiotic bacteria using confocal microscopy and
Sarah Moore1, Kasipathy Kailasapathy1, Michael Phillips1
1Science Department, School of Health & Science, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
Abstract:
Microencapsulation is proposed to protect probiotic strains from food processing procedures and to maintain probiotic viability. Little research has described the in situ viability of microencapsulated probiotics. This study successfully developed a real-time viability standard curve for microencapsulated bacteria using confocal microscopy, fluorescent dyes and image analysis software.
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