Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

123
The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
123
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

318
Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
318
Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

6.8K
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
6.8K
Renal Corpuscle01:20

Renal Corpuscle

9.6K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
9.6K
Glomerular Filtration01:15

Glomerular Filtration

7.5K
The filtration membrane in the renal system is a highly specialized structure essential for filtering blood. It consists of glomerular capillaries and podocytes, forming a selective barrier that permits the passage of water and small solutes while restricting most plasma proteins and blood cells.
Components of the Filtration Membrane
The filtration process involves three key layers: the glomerular endothelial cells, the basement membrane, and the podocyte-formed filtration slits.
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Birth Weight Measurement Gaps and Global Kidney Health.

Kidney360·2026
Same author

The immunoproteasome disturbs neuronal metabolism and drives neurodegeneration in multiple sclerosis.

Cell·2026
Same author

Integrating nephron number into risk stratification for IgA nephropathy.

Clinical and experimental nephrology·2026
Same author

The Impact of Low-Protein Diet on the Molecular and Cellular Development of the Fetal Kidney.

Journal of the American Society of Nephrology : JASN·2026
Same author

Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.

Journal of hepatology·2026
Same author

Lysine-specific histone demethylase 1a regulates nephron development and long-term transcriptional programming.

JCI insight·2026
Same journal

Sex differences in renal acid-base regulation.

Current opinion in nephrology and hypertension·2026
Same journal

Primary prevention of chronic kidney disease in type 2 diabetes mellitus with sodium-glucose cotransporter 2 inhibitors.

Current opinion in nephrology and hypertension·2026
Same journal

Financial and policy challenges of delivering kidney replacement therapies in resource-limited settings.

Current opinion in nephrology and hypertension·2026
Same journal

The role of kir4.1/Kir5.1 in mediating the effect of angiotensin-II on Na-Cl-cotransporter.

Current opinion in nephrology and hypertension·2026
Same journal

Role of the calcium-sensing receptor in regulating calcium transport in the thick ascending limb.

Current opinion in nephrology and hypertension·2026
Same journal

Social determinants of chronic kidney disease: from association to clinical and population action.

Current opinion in nephrology and hypertension·2026
See all related articles

Related Experiment Video

Updated: Apr 14, 2026

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
10:14

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

14.8K

Counting glomeruli and podocytes: rationale and methodologies.

Victor G Puelles1, John F Bertram

  • 1Department of Anatomy and Developmental Biology, School of Biomedical Sciences, Faculty of Medicine, Nursing, and Health Sciences, Monash University, Melbourne, Victoria, Australia.

Current Opinion in Nephrology and Hypertension
|April 19, 2015
PubMed
Summary
This summary is machine-generated.

Accurately estimating glomeruli and podocyte numbers is crucial for understanding kidney health. Recent advancements offer improved methods for precise quantification, aiding research into kidney disease.

More Related Videos

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues
09:50

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues

Published on: February 9, 2024

2.0K
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

11.7K

Related Experiment Videos

Last Updated: Apr 14, 2026

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
10:14

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

14.8K
Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues
09:50

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues

Published on: February 9, 2024

2.0K
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

11.7K

Area of Science:

  • Nephrology
  • Renal Physiology
  • Cell Biology

Background:

  • Growing interest in glomeruli and podocyte numbers is driven by understanding nephron endowment's fetal origins.
  • Low nephron count is linked to adult chronic kidney and cardiovascular diseases.
  • The podocyte depletion hypothesis highlights the importance of these cells in kidney health.

Purpose of the Study:

  • To review current gold-standard and novel methods for estimating glomerular and podocyte numbers.
  • To discuss advancements in quantifying these cell populations, particularly in small samples.

Main Methods:

  • Gold-standard: Design-based stereology for whole kidneys or large tissue samples, ensuring accuracy by negating bias.
  • Challenges: Stereology is often tedious, time-consuming, and unsuitable for small biopsies.
  • Novel approaches: Development of high-throughput methods like MRI for glomerular number and flow cytometry for podocyte number estimation in small samples.

Main Results:

  • Accurate and precise estimation of glomerular and podocyte numbers has historically been challenging.
  • New methods are emerging to overcome limitations of traditional stereological techniques, especially for biopsies.
  • Innovations include MRI for glomerular counting and flow cytometry for podocyte quantification.

Conclusions:

  • Recent advancements have significantly improved our ability to accurately estimate glomerular and podocyte numbers.
  • These enhanced methods are vital for investigating the relationship between cell counts and kidney health/disease.
  • The review covers methods developed within the last three years, positioning researchers for better insights.