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Related Experiment Videos

Study of postmortem blood circulation.

M Gómez Zapata1, M Alcaraz, A Luna

  • 1Department of Forensic Medicine, University of Murcia, Spain.

Zeitschrift Fur Rechtsmedizin. Journal of Legal Medicine
|January 1, 1989
PubMed
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Postmortem blood redistribution affects how substances distribute in the body. Factors like heart contraction and vessel anatomy influence this, with smaller molecules like aminohippuric acid showing diffusion into urine.

Area of Science:

  • Forensic Science
  • Toxicology
  • Physiology

Background:

  • Understanding postmortem changes is crucial for interpreting forensic evidence.
  • Substance distribution after death is influenced by various physiological and physical factors.
  • Previous studies have not fully elucidated the impact of molecular weight on postmortem redistribution.

Purpose of the Study:

  • To investigate the postmortem redistribution of blood volumes.
  • To determine how molecular weight affects the distribution of injected substances.
  • To identify key factors influencing postmortem substance distribution.

Main Methods:

  • Injected substances (Lipiodol Ultrafluide, Omnitrast, aminohippuric acid, human IgG) into the left ventricle of 20 rabbits.
  • Divided rabbits into three experimental groups.

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  • Analyzed distribution of substances with different molecular weights (194.2 Da and 150,000 Da).

    • Main Results:

    • Left ventricular postmortem contraction significantly impacts blood redistribution.
    • The arterial vascular bed and vessel anatomy play a role in substance distribution.
    • Physicochemical properties, including molecular weight, influence diffusion processes.
    • Observed postmortem diffusion of aminohippuric acid into the urine.

    Conclusions:

    • Postmortem redistribution is a complex process influenced by cardiac, vascular, and molecular factors.
    • Molecular weight is a critical determinant of postmortem diffusion and distribution.
    • Findings provide insights into interpreting toxicological and forensic findings related to substance distribution after death.