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Updated: Apr 14, 2026

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
Published on: December 14, 2019
Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis
Jonathan Meade1, Patrick Bartlow2, Ram Narayan Trivedi3
1Department Chemical Engineering, Carnegie Mellon University, 15213, Pittsburgh, PA, USA. jwmeade@andrew.cmu.edu.
Abstract:
When the replication of a plasmid based on sucrose selection is deregulated via the inc1 and inc2 mutations, high copy numbers (7,000 or greater) are attained while the growth rate on minimal medium is negligibly affected. Adaptions were assumed to be required in order to sustain the growth rate. Proteomics indicated that indeed a number of adaptations occurred that included increased expression of ribosomal proteins and 2-oxoglutarate dehydrogenase. The operating space prescribed by a basic flux model that maintained phenotypic traits (e.g. growth, byproducts, etc.) within typical bounds of resolution was consistent with the flux implications of the proteomic changes.
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