Isolation of NDM-1-producing multidrug-resistant Pseudomonas putida from a paediatric case of acute gastroenteritis,

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D Bhattacharya1, S Dey1, S Kadam1

  • 1Regional Medical Research Centre (Indian Council of Medical Research, Dept. of Health Research, Govt. of India), Nehru Nagar, Belgaum, Karnataka, India.

New Microbes and New Infections
|April 21, 2015
PubMed

Insights

This study reports the first isolation of New Delhi metallo-beta-lactamase 1 (NDM-1)-producing, multidrug-resistant Pseudomonas putida from a patient with acute gastroenteritis. This rare pathogen displayed resistance to multiple antibiotics, including carbapenems.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Pseudomonas putida is typically susceptible to antibiotics, with limited data on resistance in clinical isolates.
  • Uncommon opportunistic pathogens can cause significant clinical infections.

Purpose of the Study:

  • To report the first isolation of New Delhi metallo-beta-lactamase 1 (NDM-1)-producing multidrug-resistant Pseudomonas putida.
  • To characterize the antimicrobial resistance profile and genetic basis of resistance in this rare isolate.

Main Methods:

  • Phenotypic antimicrobial susceptibility testing was performed.
  • Molecular methods were used to identify resistance genes, including bla NDM-1, and analyze quinolone resistance determining regions.

Main Results:

  • The Pseudomonas putida isolate was multidrug-resistant, exhibiting resistance to fluoroquinolones, third-generation cephalosporins, and carbapenems.
  • The isolate carried the bla NDM-1 gene and other resistance genes (qepA, bla TEM, bla OXA1, bla OXA7).
  • Multiple mutations were identified in the quinolone resistance determining region.

Conclusions:

  • This case highlights the emergence of NDM-1-producing multidrug-resistant Pseudomonas putida as a potential cause of gastroenteritis.
  • Clinicians should consider rare pathogens in cases where common bacteria are not identified.
Keywords:
16S rRNANDM-1Pseudomonas putidadiarrhoeamultidrug resistancemutation

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