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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
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A mass spectrometric-derived cell surface protein atlas.
Damaris Bausch-Fluck1, Andreas Hofmann2, Thomas Bock2
1Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; Department of Health Sciences and Technology, BMPP, ETH Zurich, Zurich, Switzerland.
Plos One
|April 21, 2015
Summary
Researchers mapped cell surface proteins across many cell types using Cell Surface Capture (CSC) technology. This created a Cell Surface Protein Atlas (CSPA) revealing quantitative differences guide cell function, aiding drug target discovery.
Area of Science:
- Proteomics
- Cell Biology
- Biotechnology
Background:
- Cell surface proteins are crucial for cell identification and drug targeting.
- Limited data exists on the complete cell surface protein repertoire (surfaceome) for individual cell types.
Purpose of the Study:
- To create a comprehensive Cell Surface Protein Atlas (CSPA) using Cell Surface Capture (CSC) technology.
- To provide high-resolution snapshots of the surfaceome for human and mouse cell types.
Main Methods:
- Applied Cell Surface Capture (CSC) technology to 41 human and 31 mouse cell types.
- Generated mass spectrometry data to identify cell surface glycoproteins.
- Developed an interactive database and data matrix for surfaceome analysis.
Main Results:
- Identified 1492 human and 1296 mouse cell surface glycoproteins with experimental evidence.
- Documented expression of 136 G-protein coupled receptors and 75 membrane receptor tyrosine-protein kinases.
- Revealed that quantitative surfaceome variations, not just qualitative ones, primarily dictate cell function.
Conclusions:
- The Cell Surface Protein Atlas (CSPA) offers valuable insights into cell surface protein expression.
- CSPA facilitates drug target evaluation, cell type classification, and understanding cell signaling in microenvironments.
