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Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
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Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
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Related Experiment Video

Updated: Apr 14, 2026

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing
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Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing

Published on: July 17, 2019

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Lipid droplet dynamics in budding yeast.

Chao-Wen Wang1

  • 1Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, 11529, Taiwan, cwwang02@gate.sinica.edu.tw.

Cellular and Molecular Life Sciences : CMLS
|April 21, 2015
PubMed
Summary

Lipid droplets (LDs) are dynamic organelles storing energy in eukaryotic cells. Recent research highlights their diverse roles in metabolism, cellular functions, and disease, particularly in budding yeast.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Eukaryotic cells store excess fatty acids as neutral lipids, primarily triacylglycerols and sterol esters, within lipid droplets (LDs).
  • LDs originate from the endoplasmic reticulum and are highly dynamic organelles involved in numerous cellular processes.
  • The breakdown of lipids within LDs fuels various metabolic pathways, including energy production, membrane synthesis, and lipid signaling.

Purpose of the Study:

  • To review recent advances in lipid droplet (LD) research.
  • To emphasize the diverse physiological roles of LDs.
  • To focus on LDs within the model system of budding yeast.

Main Methods:

  • Literature review of recent research on lipid droplets.
  • Analysis of the physiological roles of LDs in budding yeast.

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Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
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  • Synthesis of current knowledge on LD biogenesis, function, and metabolism.

    • Main Results:

    • LDs are implicated in protein degradation and pathogen infection.
    • LDs are linked to human metabolic diseases.
    • LDs show potential for biofuel production.

    Conclusions:

    • Recent research has significantly expanded our understanding of lipid droplet functions.
    • LDs play critical roles in cellular metabolism, homeostasis, and disease.
    • Budding yeast serves as a valuable model for studying diverse LD physiological roles, paving the way for future research.