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Updated: Apr 14, 2026

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
[Study on chemical constituents from active fraction of Cynanchum versicolor]
Objective:
To investigate the chemical constituents of active fraction of Cynanchum versicolor.
Methods:
The ethanol extract of Cynanchum versicolor was purified by macroporous resin, silica gel column and Sephadex LH-20 column chromatography, and structures of the isolated compounds were identified by spectroscopic analysis and comparison with those of literatures.
Results:
Six compounds were isolated and identified as glaucogenin C (I), 24-methyl-5α-cholesta-7,22-diene-3β,5,6β-triol (II), syringic acid (III), 3,4-dihydroxyacetophenone (IV), 4-hydroxyacetophenone (V), and 4-hyroxy-3-methoxyacetophenone (VI).
Conclusion:
Compounds I, II and IV are isolated from this plant for the first time.
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