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Updated: Apr 14, 2026

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

H5 avian influenza virus pathotyping using oligonucleotide microarray.

Lih-Chiann Wang¹, Dean Huang¹, Ming-Chu Cheng²

¹School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.

Journal of Virological Methods

|April 22, 2015

Summary

This summary is machine-generated.

A new oligonucleotide microarray method rapidly distinguishes highly pathogenic H5 avian influenza viruses from low-pathogenicity strains. This sensitive tool offers naked-eye detection, aiding poultry industry surveillance and diagnostics.

Keywords:

Avian influenza virus Oligonucleotide microarray Pathotyping

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Last Updated: Apr 14, 2026

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Area of Science:

Veterinary Virology
Molecular Diagnostics
Biotechnology

Background:

Avian influenza virus (AIV) poses a significant threat to the global poultry industry.
Accurate and rapid differentiation of highly pathogenic (HP) and low-pathogenicity (LP) H5 AIV strains is crucial for effective disease control and surveillance.
Existing diagnostic methods may lack the speed or sensitivity required during outbreaks.

Purpose of the Study:

To develop and validate a novel, rapid molecular strategy for the pathotyping of H5 avian influenza virus.
To enable the differentiation between HP and LP H5 AIV strains using a sensitive and accessible detection method.

Main Methods:

Development of an oligonucleotide microarray targeting the hemagglutinin (HA) gene specific to H5 AIV.
Detection of the motif at the HA precursor protein cleavage site to assess pathogenicity.
Utilized 13 specific microarray probes for differentiating HP and LP H5 AIV strains in Taiwan.

Main Results:

The oligonucleotide microarray successfully differentiated between HP and LP H5 AIV strains with naked-eye visualization.
Achieved a remarkable detection limit of 3.4 viral RNA copies, surpassing the sensitivity of traditional reverse transcription polymerase chain reaction (RT-PCR) by 1000-fold.
Demonstrated the potential for rapid H5 pathogenicity determination.

Conclusions:

The developed oligonucleotide microarray provides a rapid, sensitive, and visually interpretable method for H5 avian influenza virus pathotyping.
This technology offers a valuable alternative for H5 pathogenicity determination, particularly for laboratories with limited resources or facilities.
Facilitates enhanced surveillance and diagnostic capabilities for H5 AIV in the poultry sector.