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Voltammetric studies of topotecan transfer across liquid/liquid interfaces and sensing applications

Hye Rim Kim1, Carlos M Pereira2, Hye Youn Han1

  • 1†Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu-city, 702-701, Republic of Korea.

Analytical Chemistry
|April 22, 2015
PubMed

Insights

A novel amperometric sensor was developed for detecting topotecan, an anticancer drug. This sensor utilizes a microhole gel interface for sensitive and selective quantification in biological samples.

Area of Science:

  • Electroanalytical Chemistry
  • Chemical Sensors
  • Pharmaceutical Analysis

Background:

  • Topotecan is a crucial water-soluble anticancer drug for treating ovarian and lung cancers.
  • Accurate quantification of topotecan is essential for effective cancer therapy.
  • Existing analytical methods may require complex sample preparation or lack sensitivity.

Purpose of the Study:

  • To develop a novel amperometric ion sensor for the quantitative analysis of topotecan.
  • To investigate the electrochemical behavior of topotecan at a water/organic gel interface.
  • To establish a sensitive, selective, and practical method for topotecan detection.

Main Methods:

  • Development of a microhole-supported water/polyvinyl chloride-2-nitrophenyloctyl ether (PVC-NPOE) gel interface sensor.
  • Voltammetric analysis including cyclic voltammetry and differential pulse stripping voltammetry (DPSV).
  • Investigation of topotecan transfer across the polarized interface and optimization of detection parameters.

Main Results:

  • The sensor demonstrated a linear response to topotecan concentration.
  • Differential pulse stripping voltammetry (DPSV) with preconcentration in the gel layer enhanced sensitivity.
  • A low detectable concentration of 0.1 μM was achieved with good selectivity.
  • The sensor was successfully applied to analyze topotecan in diluted serum samples.

Conclusions:

  • A highly sensitive and selective amperometric sensor for topotecan was successfully developed.
  • The developed sensor offers a promising alternative for topotecan quantification in complex matrices.
  • The method shows potential for clinical and pharmaceutical applications, comparable to HPLC.

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