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Updated: Apr 14, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
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The p53/miR-34a/SIRT1 Positive Feedback Loop in Quercetin-Induced Apoptosis

Guohua Lou1, Yanning Liu, Shanshan Wu

  • 1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University. Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|April 22, 2015
PubMed
Abstract

Insights

Quercetin

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Biochemistry

Background:

  • Quercetin exhibits anti-tumor properties, but its molecular mechanisms require further investigation.
  • Understanding the role of microRNAs (miRNAs) in quercetin's anticancer effects is crucial.

Purpose of the Study:

  • To investigate the role of miRNA in mediating the anticancer effects of quercetin.
  • To elucidate the molecular pathways involved in quercetin's anti-tumor activity.

Main Methods:

  • Microarray analysis to detect differential miRNA expression in HepG2 and Huh7 cells treated with quercetin.
  • xCELLigence, flow cytometry, RT-PCR, and Western blot assays to assess cell proliferation, apoptosis, cell cycle, and gene/protein expression.

Main Results:

  • miR-34a was upregulated in quercetin-treated HepG2 cells with wild-type p53.
  • Inhibiting miR-34a reduced quercetin sensitivity, increased SIRT1 expression, and decreased p53 acetylation and p53-related gene expression.

Conclusions:

  • miR-34a is a key mediator of quercetin's anti-tumor effects in hepatocellular carcinoma (HCC).
  • A p53/miR-34a/SIRT1 feedback loop enhances apoptosis signaling, promoting cell death in HCC.

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