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Published on: February 8, 2016
Effective inactivation of Saccharomyces cerevisiae in minimally processed Makgeolli using low-pressure
1Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701 Republic of Korea ; Institute of Advanced Machinery and Design, Department of Mechanical and Aerospace Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744 Republic of Korea.
Abstract:
In order to address the limitations associated with the inefficient pasteurization platform used to make Makgeolli, such as the presence of turbid colloidal dispersions in suspension, commercially available Makgeolli was minimally processed using a low-pressure homogenization-based pasteurization (LHBP) process. This continuous process demonstrates that promptly reducing the exposure time to excessive heat using either large molecules or insoluble particles can dramatically improve internal quality and decrease irreversible damage. Specifically, optimal homogenization increased concomitantly with physical parameters such as colloidal stability (65.0% of maximum and below 25-μm particles) following two repetitions at 25.0 MPa. However, biochemical parameters such as microbial population, acidity, and the presence of fermentable sugars rarely affected Makgeolli quality. Remarkably, there was a 4.5-log reduction in the number of Saccharomyces cerevisiae target cells at 53.5°C for 70 sec in optimally homogenized Makgeolli. This value was higher than the 37.7% measured from traditionally pasteurized Makgeolli. In contrast to the analytical similarity among homogenized Makgeollis, our objective quality evaluation demonstrated significant differences between pasteurized (or unpasteurized) Makgeolli and LHBP-treated Makgeolli. Low-pressure homogenization-based pasteurization, Makgeolli, minimal processing-preservation, Saccharomyces cerevisiae, suspension stability.
Insights
Low-pressure homogenization-based pasteurization (LHBP) improves Makgeolli quality by reducing heat exposure. This minimal processing enhances colloidal stability and microbial reduction of Saccharomyces cerevisiae.
Area of Science:
- Food Science
- Microbiology
- Process Engineering
Background:
- Traditional Makgeolli pasteurization faces limitations due to inefficient heat exposure, affecting product quality.
- Turbid colloidal dispersions in suspension are a common issue in commercially produced Makgeolli.
Purpose of the Study:
- To investigate the efficacy of low-pressure homogenization-based pasteurization (LHBP) as a minimal processing technique for Makgeolli.
- To improve the internal quality and reduce irreversible damage in Makgeolli through optimized homogenization.
Main Methods:
- Commercially available Makgeolli was minimally processed using a continuous low-pressure homogenization-based pasteurization (LHBP) process.
- Optimal homogenization parameters were determined, involving two repetitions at 25.0 MPa.
- The impact of LHBP on physical (colloidal stability, particle size) and biochemical (microbial population, acidity, sugars) parameters was assessed.
Main Results:
- Optimal homogenization significantly improved colloidal stability, achieving 65.0% of maximum with particles below 25 μm.
- LHBP resulted in a 4.5-log reduction of Saccharomyces cerevisiae at 53.5°C for 70 sec, surpassing traditional pasteurization.
- Biochemical parameters showed minimal changes, indicating preservation of Makgeolli's characteristics.
Conclusions:
- Low-pressure homogenization-based pasteurization (LHBP) is an effective minimal processing method for enhancing Makgeolli quality.
- LHBP significantly improves suspension stability and microbial inactivation while preserving biochemical integrity.
- This technique offers a promising alternative to traditional pasteurization for Makgeolli production.
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