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Monoclonal Antibodies Against NS2B of Japanese Encephalitis Virus
Qian Dong1, Qiuping Xu, Xindi Ruan
1State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, Hubei, China .
Abstract:
Japanese encephalitis (JE) is one of the most important viral encephalitis, caused by the Japanese encephalitis virus (JEV). The function of non-structural protein 2B (NS2B) mostly remains unclear. In our study, NS2B of Japanese encephalitis virus (JEV) was expressed in Escherichia coli and purified by dialysis. After fusing mouse myeloma cell line SP2/0 with spleen lymphocytes from NS2B protein immunized mice, three clones of monoclonal antibodies (MAbs), named 1B9, 3E12, and 4E6, were generated. The specificity and sensitivity of MAbs were demonstrated by ELISA, indirect immunofluorescence assay, and Western blot. These MAbs will be useful in further exploration of the functions of NS2B and the pathogenesis of Japanese encephalitis virus.
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