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Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy
Published on: May 22, 2020
Direct delivery of a cytotoxic anticancer agent into the metastatic lymph node using nano/microbubbles and ultrasound
Takuma Sato1, Shiro Mori2, Maya Sakamoto3
1Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo-machi, Aoba, Sendai, Miyagi, 980-8575, Japan; Department of Urology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba, Sendai, Miyagi, 980-8575, Japan.
Abstract:
Direct injection of an anticancer agent into a metastatic lymph node (LN) has not been used as a standard treatment because evidence concerning the efficacy of local administration of a drug into a metastatic LN has not been established. Here we show that the combination of intralymphatic drug delivery with nano/microbubbles (NMBs) and ultrasound has the potential to improve the chemotherapeutic effect. We delivered cis-diamminedichloroplatinum (II) (CDDP) into breast carcinoma cells in vitro and found that apoptotic processes were involved in the antitumor action. Next, we investigated the antitumor effect of intralymphatic chemotherapy with NMBs and ultrasound in an experimental model of LN metastasis using MXH10/Mo-lpr/lpr mice exhibiting lymphadenopathy. The combination of intralymphatic chemotherapy with NMBs and ultrasound has the potential to improve the delivery of CDDP into target LNs without damage to the surrounding normal tissues. The present study indicates that intralymphatic drug delivery with NMBs and ultrasound will potentially be of great benefit in the clinical setting.
Insights
Intralymphatic chemotherapy using nano/microbubbles and ultrasound enhances anticancer drug delivery to metastatic lymph nodes. This novel approach shows potential for improved treatment efficacy without damaging surrounding tissues.
Area of Science:
- Oncology
- Nanotechnology
- Medical Imaging
Background:
- Direct injection of anticancer agents into metastatic lymph nodes (LNs) is not a standard treatment due to insufficient evidence of efficacy.
- Local drug administration into metastatic LNs requires improved delivery methods to enhance chemotherapeutic effects.
Purpose of the Study:
- To evaluate the potential of intralymphatic drug delivery combined with nano/microbubbles (NMBs) and ultrasound to improve chemotherapy in metastatic lymph nodes.
- To investigate the antitumor action of cis-diamminedichloroplatinum (II) (CDDP) in breast carcinoma cells and its efficacy in a mouse model of LN metastasis.
Main Methods:
- In vitro delivery of CDDP into breast carcinoma cells to assess apoptotic processes.
- Intralymphatic chemotherapy using NMBs and ultrasound in MXH10/Mo-lpr/lpr mice with lymphadenopathy to model LN metastasis.
Main Results:
- CDDP delivery into breast carcinoma cells induced apoptotic processes, indicating antitumor action.
- The combination of intralymphatic chemotherapy with NMBs and ultrasound improved CDDP delivery to target LNs.
- This method demonstrated potential for improved drug delivery without damaging surrounding normal tissues.
Conclusions:
- Intralymphatic drug delivery with NMBs and ultrasound enhances chemotherapeutic agent delivery to metastatic lymph nodes.
- This approach shows promise for improving the efficacy of cancer treatment in the clinical setting.
- The technique offers a potential benefit for patients with lymph node metastasis.
