[Influence of microcystin-LR on cell viability and surface characteristics of Pseudomonas putida]
Abstract:
In microcystin-LR (MC-LR) degradation system, the change in surface characteristics and cell viability of Pseudomonas putida was studied. The purpose of this study was to reveal the influence of MC-LR on P. putida and elucidate the toxicity of MC-LR on microorganisms. The result demonstrated that MC-LR enhanced the cytoplasmic membrane permeability, as well as affected the ion metabolism and protein release of P. putida. The soluble sugar and Na+, Cl-release increased with the rising concentration of MC-LR ranging from 0 mg x L(-1) to 2.0 mg x L(-1). Flow Cytometry Method(FCM) analysis revealed that MC-LR accelerated the death of P. putida, and the death rate increased with the ascending concentration of MC-LR. Compared with the control, the death rate on day 5 increased by nearly 30% when 2.5 mg x L(-1) MC-LR was added. Scanning electron microscopy (SEM) analysis showed that the cells were deformed under the toxicity of MC-LR. After 5-day exposure to 2.5 mg x L(-1) MC-LR, the majority of the cells were ruptured and the intracellular materials flew out. The cellular structure was severely damaged under this condition.
Insights
Microcystin-LR (MC-LR) significantly harms Pseudomonas putida, increasing cell death and damaging cellular structures. This study reveals MC-LR
Area of Science:
- Environmental Microbiology
- Toxicology
- Bioremediation
Background:
- Microcystin-LR (MC-LR) is a potent cyanotoxin impacting aquatic ecosystems.
- Understanding MC-LR's effects on key microorganisms is crucial for risk assessment.
- Pseudomonas putida plays a role in biodegradation processes.
Purpose of the Study:
- To investigate the impact of MC-LR on the surface characteristics and viability of Pseudomonas putida.
- To elucidate the toxic mechanisms of MC-LR on microbial cells.
- To assess MC-LR's influence within a degradation system.
Main Methods:
- Exposure of Pseudomonas putida to varying concentrations of MC-LR.
- Analysis of cytoplasmic membrane permeability and ion metabolism.
- Flow Cytometry (FCM) for cell viability assessment.
- Scanning Electron Microscopy (SEM) for morphological changes.
Main Results:
- MC-LR increased cytoplasmic membrane permeability, soluble sugar release, and Na+, Cl- ion efflux.
- MC-LR accelerated P. putida cell death, with a dose-dependent increase in mortality.
- SEM revealed cell deformation, rupture, and severe structural damage upon MC-LR exposure.
Conclusions:
- MC-LR exhibits significant toxicity towards Pseudomonas putida.
- The observed effects include compromised membrane integrity, altered ion balance, and cell lysis.
- These findings highlight the detrimental impact of MC-LR on microbial communities in contaminated environments.
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