Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.9K
RNA Interference01:23

RNA Interference

28.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.8K
RNA Interference01:23

RNA Interference

7.9K
7.9K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.1K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.1K
Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K
MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Zinc Adaptation on Histological Morphology, Antioxidant Responses, and Expression of Immune-Related Genes of Grass Carp (Ctenopharyngodon idella).

Biological trace element research·2022
Same author

Effect of Spirulina and Ferrous Fumarate on Intestinal Morphology and the Diversity of Gut Microbiota of Yellow River Carp.

Biological trace element research·2021
Same author

Discovery and characterization of conserved and novel microRNAs from blunt snout bream (Megalobrama amblycephala) by deep sequencing.

Gene·2018
Same author

Characterization of conserved and novel miRNAs using deep sequencing and prediction of miRNA targets in Crucian carp (Carassius auratus).

Gene·2017
Same author

Identification and characterization of the Chinese giant salamander (Andrias davidianus) miRNAs by deep sequencing and predication of their targets.

3 Biotech·2017
Same author

Identification and characterization of known and novel microRNAs in three tissues of Chinese giant salamander base on deep sequencing approach.

Genomics·2017
Same journal

[The Point Mutation in NOGGIN2 Protein That Enhances Its Ability to Bind Activin].

Bioorganicheskaia khimiia·2016
Same journal

[The Cytoskelrtal Protein Zvxin Interacts with the Hedgehog Receptor Patched].

Bioorganicheskaia khimiia·2016
Same journal

[Synthesis and Neurotropic Activity of 2,4-pyrano[4',3':4,5]pyrido[2,3-b]thieno[3,2-d] Pyrimidines].

Bioorganicheskaia khimiia·2016
Same journal

[Inhibitory Properties of Nitrogen-Containing Adamantane Derivatives with Monoterpenoid Fragments Against Tyrosyl-DNA Phosphodiesterase I].

Bioorganicheskaia khimiia·2016
Same journal

[Modulation of Ca(2+)-Dependent Proteiolysis under the Action of Weak Low-Frequency Magnetic Fields].

Bioorganicheskaia khimiia·2016
Same journal

[Intracellular Protein Degradation in Growth of Atlantic Salmon, Salmo salar L].

Bioorganicheskaia khimiia·2016
See all related articles

Related Experiment Video

Updated: Apr 14, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

8.1K

PiRNAs biogenesis and its functions.

Yong Huang, Jun Yan Bai, Hong Tao Ren

    Bioorganicheskaia Khimiia
    |April 23, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Small non-coding RNAs called piRNAs (piwi-interacting RNAs) are crucial for germline DNA integrity, transposon silencing, and epigenetic regulation. This review covers recent advances in piRNA biogenesis and function across eukaryotes.

    More Related Videos

    In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
    10:27

    In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

    Published on: October 21, 2022

    2.2K
    Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
    11:00

    Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

    Published on: June 12, 2018

    14.8K

    Related Experiment Videos

    Last Updated: Apr 14, 2026

    MicroRNA-based Regulation of Picornavirus Tropism
    09:05

    MicroRNA-based Regulation of Picornavirus Tropism

    Published on: February 6, 2017

    8.1K
    In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
    10:27

    In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

    Published on: October 21, 2022

    2.2K
    Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
    11:00

    Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

    Published on: June 12, 2018

    14.8K

    Area of Science:

    • Molecular Biology
    • Genetics
    • Epigenetics

    Background:

    • piRNAs (piwi-interacting RNAs) are small non-coding RNAs (26-33 nt).
    • They interact with piwi proteins in the Argonaute family.
    • piRNAs are vital for various cellular processes.

    Purpose of the Study:

    • To review recent research on piRNA biogenesis.
    • To summarize the diverse functions of piRNAs in eukaryotes.
    • To highlight the role of piRNAs in genetic regulation.

    Main Methods:

    • Literature review of recent studies on piRNAs.
    • Analysis of piRNA biogenesis pathways.
    • Examination of piRNA-mediated gene silencing mechanisms.

    Main Results:

    • piRNAs are involved in embryonic development and germline DNA maintenance.
    • They play a key role in silencing transposable elements.
    • piRNAs contribute to heterochromatin formation and epigenetic sex determination.

    Conclusions:

    • piRNA biogenesis and function are complex and essential in eukaryotes.
    • Further research is needed to fully elucidate piRNA pathways.
    • piRNAs represent a significant regulatory layer in genome stability and gene expression.