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Updated: Apr 14, 2026

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Fabrication of 3-dimensional multicellular microvascular structures.

Sebastian F Barreto-Ortiz¹, Jamie Fradkin¹, Joon Eoh¹

¹*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology

|April 23, 2015

Summary

Researchers developed 3D microfibers using hydrogel electrospinning to create microvasculature. This method enhances extracellular matrix deposition, enabling the formation of self-supporting, multilayered microvascular structures for regenerative medicine applications.

Keywords:

endothelial cell extracellular matrix fibrin microfiber perivascular cell

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Last Updated: Apr 14, 2026

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

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Published on: January 3, 2017

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Area of Science:

Biomaterials Engineering
Vascular Biology
Tissue Engineering

Background:

Limited research exists on developing microvasculature, the critical link between larger blood vessels and capillary beds.
Current tissue engineering strategies have focused on larger vessels or capillary networks, neglecting the intermediate microvasculature.
Developing functional microvasculature is essential for creating viable engineered tissues and organs.

Purpose of the Study:

To evaluate the efficacy of 3D microfibers fabricated by hydrogel electrospinning as templates for microvascular structure formation.
To investigate whether 3D microfibers enhance extracellular matrix (ECM) deposition by vascular cells.
To engineer freestanding, luminal multicellular microvasculature.

Main Methods:

Fabrication of 3D microfibers using hydrogel electrospinning.
Culture of vascular cells (endothelial colony-forming cells, pericytes, vascular smooth muscle cells) on fibrin microfibers.
Assessment of ECM deposition using confocal microscopy and RT-PCR.
Analysis of microvascular structure and lumen formation after microfiber core removal.

Main Results:

Fibrin microfibers significantly increased ECM protein deposition by vascular cells compared to 2D cultures.
Deposited ECM proteins included collagen types I, III, and IV, elastin, fibronectin, and laminin, forming layers of the vascular wall.
Successfully engineered multicellular microvascular structures with organized endothelium and a perivascular tunica media.
Achieved self-supporting, multilayered microvasculature with a distinct circular lumen post-core removal.

Conclusions:

3D hydrogel electrospun microfibers serve as effective templates for engineering microvasculature.
This approach promotes enhanced ECM deposition and the formation of organized, self-supporting microvascular structures.
The method advances the development of human microvasculature for both basic research and translational applications in regenerative medicine.