Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Labeling DNA Probes03:31

Labeling DNA Probes

9.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancement in catalytic activity of CotA-laccase from Bacillus pumilus W3 via site-directed mutagenesis.

Journal of bioscience and bioengineering·2019
Same author

Characteristics and evaluation of urban soundscapes worthy of preservation.

Journal of environmental management·2019
Same author

A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.

The Journal of experimental medicine·2019
Same author

[Determination of pesticide residues in wolfberry using QuEChERS-gas chromatography-tandem mass spectrometry].

Se pu = Chinese journal of chromatography·2019
Same author

Amentoflavone induces cell cycle arrest, apoptosis, and autophagy in BV-2 cells.

Frontiers in bioscience (Landmark edition)·2019
Same author

Biosynthesis of Novel Shikonin Glucosides by Enzymatic Glycosylation.

Chemical & pharmaceutical bulletin·2019
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 14, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.9K

Decoding of quantum dots encoded microbeads using a hyperspectral fluorescence imaging method.

Yixi Liu1, Le Liu2, Yonghong He1

  • 1‡Department of Physics, Tsinghua University, Beijing 100084, China.

Analytical Chemistry
|April 23, 2015
PubMed
Summary
This summary is machine-generated.

We developed a hyperspectral fluorescence imaging (HFI) method to decode quantum dot-encoded microbeads. This advanced HFI system offers higher spectral resolution for improved biological and medical applications.

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.4K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.7K

Related Experiment Videos

Last Updated: Apr 14, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.9K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.4K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.7K

Area of Science:

  • Biomedical Engineering
  • Spectroscopy
  • Nanotechnology

Background:

  • Quantum dot (QD)-encoded microbeads are valuable for multiplexed assays.
  • Accurate decoding of QD fluorescence spectra is crucial for assay performance.
  • Existing methods may lack sufficient spectral resolution or spatial information.

Purpose of the Study:

  • To present a novel decoding method for QD-encoded microbeads using line scan hyperspectral fluorescence imaging (HFI).
  • To develop an HFI system capable of capturing both fluorescence spectra and spatial information of microbeads.
  • To evaluate the performance of the HFI system compared to traditional flow cytometry.

Main Methods:

  • Development of a line scan hyperspectral fluorescence imaging (HFI) system.
  • Implementation of a decoding scheme for multicolor microbead spectra.
  • Comparative analysis of HFI system and flow cytometry.
  • Characterization of microbead surface modification (polydopamine) using scanning electron microscopy (SEM).
  • Verification of single-stranded DNA (ssDNA) immobilization using laser confocal microscopy.

Main Results:

  • The HFI system achieved higher spectral resolution than flow cytometry, enabling more spectral channels.
  • Successful detection and decoding of ssDNA-immobilized multicolor beads using the HFI system.
  • Demonstrated the efficiency of the HFI system for analyzing QD-encoded microbeads.
  • Confirmed successful surface modification and ssDNA immobilization on microbeads.

Conclusions:

  • The developed HFI system provides a powerful tool for decoding QD-encoded microbeads with enhanced spectral resolution.
  • The HFI method is efficient and suitable for analyzing biological samples, showing potential for practical applications.
  • This technology can advance multiplexed biological and medical diagnostics.