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[Isolation of alpha-latrotoxin from karakurt spider venom using monoclonal antibodies]
Bioorganicheskaia Khimiia
|September 1, 1989
Abstract:
A method of alpha-latrotoxin (LT) isolation from the venom of Latrodectus mactans tredecimguttatus by means of immunoaffinity chromatography on sepharose conjugated with monoclonal antibodies against LT has been developed. This one-step, high-yield, relatively simple and rapid procedure yields active LT for structural and functional studies of its receptor.