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Toxicological Effect of Manganese on NF-κB/iNOS-COX-2 Signaling Pathway in Chicken Testes

Ye Du1, Yihao Zhu1, Xiaojie Teng2

  • 1College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.

Biological Trace Element Research
|April 24, 2015
PubMed

Insights

Manganese (Mn) poisoning causes inflammatory damage in chicken testes by activating the NF-κB/iNOS-COX-2 pathway. This study reveals Mn-induced testicular injury in poultry, impacting reproductive health.

Area of Science:

  • Veterinary Toxicology
  • Molecular Biology
  • Poultry Science

Background:

  • Manganese (Mn) pollution is a known cause of organ dysfunction.
  • While Mn toxicity in poultry is documented, its specific impact on testicular inflammation is unreported.
  • Understanding Mn's effects on reproductive tissues is crucial for poultry health.

Purpose of the Study:

  • To investigate the effects of Mn poisoning on the NF-κB/iNOS-COX-2 signaling pathway in chicken testes.
  • To determine the dose- and time-dependent impacts of Mn exposure on testicular tissue.
  • To elucidate the molecular mechanisms underlying Mn-induced testicular injury in poultry.

Main Methods:

  • Hyline male chickens were fed diets with varying MnCl2 concentrations (600, 900, 1800 mg/kg) for 30, 60, and 90 days.
  • Assessed mRNA expression of NF-κB, TNF-α, COX-2, and iNOS.
  • Measured nitric oxide (NO) content, iNOS activity, and performed histopathological examination of testicular tissues.

Main Results:

  • Excess Mn significantly upregulated mRNA expression of NF-κB, COX-2, TNF-α, and iNOS.
  • Increased NO content and iNOS activity were observed in a dose- and time-dependent manner.
  • Mn exposure led to dose- and time-dependent histological damage in chicken testes, indicating inflammatory injury.

Conclusions:

  • Mn exposure induces inflammatory injury in chicken testicular tissue.
  • The NF-κB/iNOS-COX-2 signaling pathway is implicated in Mn-induced testicular damage in poultry.
  • Findings highlight the detrimental effects of Mn pollution on male reproductive health in chickens.
Keywords:
ChickenManganeseNF-κB/iNOS-COX-2 signaling pathwayTestis

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