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Related Concept Videos

Hair Cells01:22

Hair Cells

46.8K
Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here.
46.8K

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Updated: Apr 14, 2026

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
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Gene Expression by Mouse Inner Ear Hair Cells during Development.

Déborah I Scheffer1, Jun Shen2, David P Corey3

  • 1Department of Neurobiology and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, Department of Otology and Laryngology, Harvard Medical School and Massachusetts Eye and Ear, Boston, Massachusetts 02114, and.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|April 24, 2015
PubMed
Summary

Researchers developed a new method to isolate inner ear hair cells, enabling a detailed study of gene expression. This research identified novel genes linked to hearing and balance, advancing our understanding of hereditary deafness.

Keywords:
FACSRNA-Seqcochleadevelopmenthair cellvestibule

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Area of Science:

  • Oto-neuroscience
  • Genomics
  • Developmental Biology

Background:

  • Inner ear hair cells are crucial for hearing and balance.
  • Pathogenic variants in hair cell genes frequently cause hereditary deafness.
  • Studying hair cell molecular biology is challenging due to their scarcity and difficulty in isolation.

Purpose of the Study:

  • To develop a protocol for isolating pure inner ear hair cells.
  • To conduct a comprehensive, cell type-specific RNA-sequencing study of gene expression during mouse inner ear development.
  • To identify novel genes expressed in hair cells and their potential role in hereditary deafness.

Main Methods:

  • Developed a fluorescence-activated cell sorting (FACS) protocol for hair cell isolation.
  • Performed RNA-sequencing (RNA-Seq) on isolated cochlear and utricular hair cells and surrounding cells from embryonic day 16 (E16) to postnatal day 7 (P7).
  • Analyzed cell type-specific gene expression patterns during inner ear development.

Main Results:

  • Achieved nearly pure populations of hair cells and surrounding cells.
  • Identified novel hair cell genes with distinct expression patterns, including vestibular-specific, cochlear-specific, and developmentally regulated genes.
  • Confirmed high expression of known hereditary deafness genes in hair cells compared to surrounding cells.

Conclusions:

  • The developed FACS protocol enables effective isolation of inner ear hair cells for molecular studies.
  • Gene expression profiling reveals new insights into hair cell development and function.
  • Genes highly expressed in hair cells are strong candidates for novel hereditary deafness genes.