Adapting for endocytosis: roles for endocytic sorting adaptors in directing neural development

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Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
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Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

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Chan Choo Yap1, Bettina Winckler1

  • 1Department of Neuroscience, University of Virginia Charlottesville, VA, USA.

Frontiers in Cellular Neuroscience
|April 24, 2015
PubMed

Insights

Regulated endocytosis controls guidance receptor levels crucial for neural development. Endocytic accessory proteins (EAPs) like Numb and Disabled regulate receptor trafficking and signaling.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Cortical development relies on precise guidance receptor and adhesion molecule function.
  • Surface levels and localization of these molecules critically influence neural guidance.
  • Receptor trafficking, including endocytosis, is vital for proper neural development outcomes.

Purpose of the Study:

  • To discuss the cell biology of regulated endocytosis in neural development.
  • To highlight the role of endocytic accessory proteins (EAPs) in receptor trafficking.
  • To examine how EAPs regulate endocytosis and post-endocytic trafficking of guidance receptors.

Main Methods:

  • Review of cell biology principles governing regulated endocytosis.
  • Focus on specific EAPs, including Numb and Disabled.
  • Analysis of EAP regulation of cognate receptors such as Notch, TrkB, and APP family members.

Main Results:

  • Regulated endocytosis quantitatively and qualitatively impacts downstream signaling pathways.
  • EAPs are key regulators of endocytosis and subsequent trafficking of guidance receptors.
  • Dysregulation of these processes can significantly affect neural development.

Conclusions:

  • Controlled endocytosis of guidance receptors is essential for proper neural development.
  • EAPs play a critical role in mediating these endocytic and trafficking events.
  • Understanding these mechanisms provides insight into ensuring accurate neural patterning and connectivity.
Keywords:
TrkBclathrindisableddoublecortinmigrationnumbsignaling endosomes

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