Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

67.1K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
67.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "A probabilistic approach to evaluate salivary microbiome in forensic science when the defense says: 'It is my twin brother'" [Forensic Sci. Int. Genet. 57, 102638].

Forensic science international. Genetics·2022
Same author

A probabilistic approach to evaluate salivary microbiome in forensic science when the Defense says: `It is my twin brother'.

Forensic science international. Genetics·2021
Same author

Pancreatitis, hypereosinophilia and bilateral pulmonary infiltrates as presentation of acute Q fever.

New microbes and new infections·2021
Same author

The dark side of SARS-CoV-2 rapid antigen testing: screening asymptomatic patients.

New microbes and new infections·2021
Same author

Universal admission screening strategy for COVID-19 highlighted the clinical importance of reporting SARS-CoV-2 viral loads.

New microbes and new infections·2020
Same author

Evaluation of the performance of rapid tests for screening carriers of acquired ESBL-producing Enterobacterales and their impact on turnaround time.

The Journal of hospital infection·2020
Same journal

Synergistic potential of probiotics and bacteriophages in combating multidrug-resistant microbial infections: A novel therapeutic strategy for the post-antibiotic era.

New microbes and new infections·2026
Same journal

FIFA World Cup 2026 ready, infection steady: A call for coordinated prevention.

New microbes and new infections·2026
Same journal

Beyond the viral triad: hantavirus cardiopulmonary syndrome as a blind spot in Brazil's post-pandemic respiratory diagnostic reasoning.

New microbes and new infections·2026
Same journal

Coexistence of endometriosis and human papilloma virus: A systematic review and meta-analysis.

New microbes and new infections·2026
Same journal

Neutrophil-to-lymphocyte ratio as a biomarker in erythema nodosum leprosum: A systematic review and meta-analysis.

New microbes and new infections·2026
Same journal

Updating specificity for the urgent design of an Andes hantavirus RT-qPCR assay.

New microbes and new infections·2026
See all related articles

Related Experiment Video

Updated: Apr 14, 2026

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.8K

Malaria real-time PCR: correlation with clinical presentation.

L Dormond1, K Jaton1, S de Vallière2

  • 1Institute of Microbiology, University Hospital Centre and University of Lausanne, Lausanne, Switzerland.

New Microbes and New Infections
|April 24, 2015
PubMed
Summary
This summary is machine-generated.

Quantitative PCR can predict Plasmodium falciparum malaria severity. Higher parasite loads detected by microscopy or PCR strongly correlate with clinical severity in patients.

Keywords:
DiagnosisPlasmodium falciparumdisease severitymalariamicroscopyreal-time PCR

More Related Videos

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
10:50

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

8.6K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.2K

Related Experiment Videos

Last Updated: Apr 14, 2026

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.8K
Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
10:50

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

8.6K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.2K

Area of Science:

  • Malariology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Plasmodium falciparum is a major cause of malaria-related mortality.
  • Accurate assessment of malaria severity is crucial for timely clinical intervention.
  • Microscopy is the standard for parasite quantification, but quantitative PCR offers higher sensitivity.

Purpose of the Study:

  • To compare the diagnostic utility of microscopy and quantitative PCR (qPCR) in assessing Plasmodium falciparum parasite load.
  • To determine the correlation between parasite load and clinical severity based on WHO criteria.
  • To evaluate the potential of qPCR in predicting malaria patient outcomes.

Main Methods:

  • A cohort of 112 patients with Plasmodium falciparum-only infections was studied.
  • Parasite density was assessed using both traditional microscopy and quantitative PCR.
  • Clinical severity was evaluated using established World Health Organization (WHO) criteria.

Main Results:

  • A significant positive correlation was observed between clinical severity and parasite load, regardless of the quantification method used (microscopy and qPCR, p < 0.001).
  • Quantitative PCR demonstrated a strong association with clinical severity, similar to microscopy.
  • Higher parasite loads, as detected by both methods, were indicative of more severe disease.

Conclusions:

  • Quantitative PCR is a valuable tool for assessing Plasmodium falciparum parasite burden.
  • The strong correlation between parasite load (measured by qPCR) and clinical severity suggests its utility in predicting patient outcomes.
  • qPCR may offer a more sensitive and predictive method for malaria severity assessment compared to microscopy.