Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Two qualitatively different structuro-functional states of mitochondria].

I P Krasinskaia, I S Litvinov, S D Zakharov

    Biokhimiia (Moscow, Russia)
    |September 1, 1989
    PubMed
    Summary

    Mitochondrial membrane proteins aggregate in low tonicity and cool temperatures, deaggregating as temperature rises. This swelling process may mimic hormone signaling in vivo.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Concerning the enigmatic cytochrome b-559 of oxygenic photosynthesis.

    Photosynthesis research·2022
    Same author

    Nonphosphorylating Oxidation in Mitochondria and Related Processes.

    Biochemistry. Biokhimiia·2021
    Same author

    Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet.

    Biochemistry. Biokhimiia·2020
    Same author

    Analysis of the Healthy Subject Response to Prolonged Contact with Tuberculosis Patients.

    Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections·2019
    Same author

    Mitochondria in Obliquely Striated Muscles of the Horsehair Worm Gordionus alpestris (Nematomorpha, Gordioidea) with Structural Organization Typical of Cells with Energy-Intensive Processes.

    Biochemistry. Biokhimiia·2019
    Same author

    On mechanisms of colicin import: the outer membrane quandary.

    The Biochemical journal·2018

    Area of Science:

    • Mitochondrial biophysics and membrane dynamics.

    Context:

    • Mitochondrial membrane fluidity and protein aggregation are influenced by tonicity and temperature.
    • Understanding these changes is crucial for elucidating mitochondrial function in cellular signaling.

    Purpose:

    • To investigate the effects of hypotonicity and temperature on mitochondrial membrane protein aggregation and phosphorylation.
    • To explore the potential role of mitochondrial swelling in hormone-induced signal transduction.

    Summary:

    • Low tonicity (120 mosM) induced an anomalous phase transition in mitochondrial membranes between 19-26°C, altering pyrene fluorescence and protein aggregation.
    • Protein deaggregation occurred above this transition temperature.
    • Decreased tonicity or increased temperature enhanced phosphorylation of a 52 kDa mitochondrial protein, suggesting a link to cellular signaling.

    Related Experiment Videos

    Impact:

    • Hypotonic swelling of mitochondria may simulate a step in hormone-induced signal transfer.
    • Findings provide insights into mitochondrial membrane dynamics and their role in cellular responses.