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Transport Function of Rice Amino Acid Permeases (AAPs).

Margaret R Taylor1, Anke Reinders1, John M Ward2

  • 1Department of Plant Biology, University of Minnesota, St. Paul, MN 55108, USA.

Plant & Cell Physiology
|April 25, 2015
PubMed
Summary
This summary is machine-generated.

Four rice amino acid permeases (AAPs) were studied. OsAAP1, OsAAP7, and OsAAP16 are general permeases, while OsAAP3 shows unique specificity for basic amino acids.

Keywords:
Xenopus oocytesamino acid transporterselectrophysiologyrice AAPs

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Area of Science:

  • Plant Molecular Biology
  • Membrane Transport
  • Biochemistry

Background:

  • Amino acid permeases (AAPs) are crucial for nutrient uptake in plants.
  • Understanding rice AAPs is vital for improving crop nutrition and yield.
  • Previous studies have characterized AAPs in model plants like Arabidopsis.

Purpose of the Study:

  • To characterize the transport function and substrate specificity of four rice amino acid permeases (OsAAP1, OsAAP3, OsAAP7, OsAAP16).
  • To determine the cellular localization of key rice AAPs.
  • To compare the function of rice AAPs with those found in Arabidopsis.

Main Methods:

  • Heterologous expression of rice AAP genes in Xenopus laevis oocytes.
  • Electrophysiological analysis to determine transport kinetics and substrate specificity.
  • Confocal microscopy using pHusion (EGFP-RFP) reporter for cellular localization studies.

Main Results:

  • OsAAP1, OsAAP7, and OsAAP16 function as general amino acid permeases.
  • OsAAP3 exhibits distinct substrate specificity, efficiently transporting basic amino acids (lysine, arginine) and excluding aromatic amino acids.
  • OsAAP1 and OsAAP3 were localized to the plasma membrane in both onion epidermal and Arabidopsis cells.

Conclusions:

  • Rice possesses diverse amino acid permeases with distinct functional roles.
  • OsAAP3 represents a specialized transporter for basic amino acids in rice.
  • Plasma membrane localization of OsAAP1 and OsAAP3 is essential for their transport function in nutrient uptake.