Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

2.5K
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<b>Special Volume of International Symposium of Tropical Fish Ecological Conservation and Biodiversity 2025 (ISTFECB 2025) Part I-Systematics, taxonomy, and species diversity of tropical fishes (Title page)</b>.

Zootaxa·2026
Same author

<b>Special Volume of International Symposium of Tropical Fish EcologicalConservation and Biodiversity 2025 (ISTFECB 2025) Part I-Systematics, taxonomy, and species diversity of tropical fishes (Table of contents)</b>.

Zootaxa·2026
Same author

<b>Preface: Special Volume of International Symposium of Tropical Fish Ecological Conservation and Biodiversity 2025 (ISTFECB 2025) Part I-Systematics, taxonomy, and species diversity of tropical fishes: contributed papers from ISTFECB 2025</b>.

Zootaxa·2026
Same author

<b>A new species of dwarf goby of <i>Trimma</i> (Teleostei: Gobiidae) from Taiping Island, South China Sea</b>.

Zootaxa·2026
Same author

<b>A new species of <i>Luciogobius</i> Gill (Teleostei: Gobiidae) from northern Taiwan</b>.

Zootaxa·2026
Same author

<b>A new wriggler of Eleotrid (Teleostei: Xenisthmidae) from Taiping Island, South China Sea</b>.

Zootaxa·2026

Related Experiment Video

Updated: Apr 14, 2026

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
10:41

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Published on: February 17, 2017

8.6K

Exploring bacterial heparinase II activities with defined substrates.

Lisa Bohlmann1, Chih-Wei Chang1, Ifor Beacham1

  • 1Institute for Glycomics, Gold Coast Campus, Griffith University, Queensland, 4222 (Australia).

Chembiochem : a European Journal of Chemical Biology
|April 25, 2015
PubMed
Summary

This study reveals distinct substrate specificities for bacterial heparinase II enzymes from Pedobacter heparinus and Bacteroides eggerthii. Understanding these differences aids in producing better low-molecular-weight heparins (LMWHs) and characterizing heparin and heparan sulfate (HS).

Keywords:
enzyme catalysisheparan sulfateheparinheparinasenatural productssubstrate specificity

More Related Videos

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

8.2K
Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.6K

Related Experiment Videos

Last Updated: Apr 14, 2026

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
10:41

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Published on: February 17, 2017

8.6K
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

8.2K
Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.6K

Area of Science:

  • Biochemistry
  • Microbiology
  • Glycobiology

Background:

  • Bacterial heparinases are crucial for generating low-molecular-weight heparins (LMWHs) and characterizing heparin and heparan sulfate (HS).
  • Heparinase II enzymes from different bacterial species exhibit varying substrate specificities, impacting their applications.

Purpose of the Study:

  • To investigate and compare the substrate specificity of heparinase II from Pedobacter heparinus and Bacteroides eggerthii.
  • To provide novel insights into the enzymatic activity and substrate preferences of these bacterial heparinases.

Main Methods:

  • Utilized well-defined heparan sulfate (HS) oligosaccharides to test enzyme activity.
  • Employed (1)H NMR spectroscopy to analyze the cleavage products and determine substrate specificity.

Main Results:

  • Heparinase II from P. heparinus demonstrated broader substrate specificity and higher activity compared to B. eggerthii heparinase II.
  • HS di- and tetrasaccharides were found to inhibit the activity of B. eggerthii heparinase II.
  • Distinct substrate specificities were identified for the two bacterial heparinase II enzymes.

Conclusions:

  • A deeper understanding of bacterial heparinase II substrate specificity is essential for optimizing LMWH production.
  • Characterizing these enzymes aids in the structural and functional analysis of heparin and HS biomolecules.
  • This research supports advancements in the therapeutic applications of heparin-derived products.