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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Apr 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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Modeling cancer processes with CRISPR-Cas9.

Xiao-Jie Lu1, Xiaolong Qi2, Dong-Hui Zheng3

  • 1Department of Gastroenterology, Shanghai East Hospital, Tongji University, School of Medicine, Shanghai, China.

Trends in Biotechnology
|April 25, 2015
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas9 gene editing technology allows for rapid modeling of cancer-related genetic changes. This facilitates extensive functional studies and the testing of new cancer therapies.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • CRISPR-Cas9 is a powerful tool for precise DNA editing.
  • Cancer involves complex genomic and epigenetic alterations.

Purpose of the Study:

  • To highlight the utility of CRISPR-Cas9 and its variants for modeling cancer-associated aberrations.
  • To emphasize its role in accelerating functional studies and therapy testing.

Main Methods:

  • Utilizing CRISPR-Cas9, nickase Cas9, and dead Cas9 systems.
  • Modeling of cancer-associated genomic and epigenetic aberrations.
  • Simultaneous or sequential aberration modeling.

Main Results:

  • CRISPR-Cas9 enables rapid and efficient modeling of complex cancer alterations.
  • Reprogrammed Cas9 variants offer versatile editing capabilities.
  • Facilitates large-scale functional interrogations and therapy screening.

Conclusions:

  • CRISPR-Cas9 technology is instrumental in advancing cancer research.
  • Enables faster and more comprehensive understanding of cancer biology.
  • Accelerates the development of novel cancer treatments.