Related Experiment Video
Updated: Apr 14, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
Published on: November 12, 2017
DNA damage induced by bare and loaded microporous coordination polymers from their ground and electronic excited
Juan G Yañuk1, María L Alomar, M Micaela Gonzalez
1IIB-INTECH (sede Chascomús)-UNSAM-CONICET. Av., Intendente Marino Km 8,2. CC 164 (7130), Chascomús, Buenos Aires, Argentina. fcabrerizo@intech.gov.ar.
Abstract:
We report on interactions of cell free double-stranded DNA (dsDNA) with a selected subgroup of Microporous Coordination Polymers (MCPs). In particular, we have studied the influence of different metal ion constituents and chemically modified linkers using a set of five benzene carboxylate-based MCPs. Our results suggest that the DNA moiety can be structurally modified in two different ways: by direct MCPs-dsDNA interaction and/or through photosensitized processes. The extent of the observed damage was found to be strongly dependent on the charge density of the material. The potential use of the MCPs tested as inert carriers of photosensitizers was demonstrated by analyzing the interaction between dsDNA and harmine-loaded Cr-based materials, both in the absence of light and upon UVA irradiation.
Related Concept Videos
Overview of DNA Repair
Chemically...
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair
Spontaneous and Induced Mutations
Long-patch Base Excision Repair
Mutations
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...

