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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

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Emerging single-cell technologies in immunology.

Jacobus Herderschee¹, Craig Fenwick¹, Giuseppe Pantaleo¹

¹*Infectious Diseases Service and Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland; and Swiss Vaccine Research Institute, Lausanne, Switzerland.

Journal of Leukocyte Biology

|April 25, 2015

Summary

This summary is machine-generated.

Single-cell technologies reveal immune cell diversity, overcoming limitations of traditional bulk methods. These advanced tools offer unprecedented detail into host-immune defense responses.

Keywords:

infectious disease sepsis vaccines

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Area of Science:

Immunology
Cell Biology

Background:

The immune system's diversity protects against pathogens.
Traditional bulk analysis methods average cellular responses, potentially missing critical single-cell contributions.
Initial immune responses are often initiated by a small subset of cells.

Purpose of the Study:

To explore the basis and outcomes of immune cell diversity.
To understand how genetically identical cells acquire distinct functions.
To review emerging single-cell technologies for immunological research.

Main Methods:

Single-cell RNA sequencing for transcriptional analysis.
Mass cytometry for high-parameter protein analysis.
Microfluidics enabling single-cell studies.

Main Results:

Single-cell technologies overcome limitations of bulk analysis.
These methods provide unprecedented detail on immune responses.
Analysis reveals functional divergence in genetically identical immune cells.

Conclusions:

Single-cell technologies are revolutionizing immunology research.
Understanding immune cell heterogeneity is crucial for host defense.
These tools offer significant advantages for basic and translational immunology.