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Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

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Quantitative microvascular hemoglobin mapping using visible light spectroscopic Optical Coherence Tomography.

Shau Poh Chong¹, Conrad W Merkle¹, Conor Leahy¹

¹Biomedical Engineering Department, University of California Davis, Davis, CA 95616, USA.

Biomedical Optics Express

|April 25, 2015

Summary

This summary is machine-generated.

This study introduces a novel method for quantifying hemoglobin concentration in biological tissues using Spectroscopic Optical Coherence Tomography (SOCT). This technique enables simultaneous mapping of oxygen saturation and total hemoglobin, advancing biomedical optics.

Keywords:

(110.4500) Optical coherence tomography (170.3880) Medical and biological imaging

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Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

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Area of Science:

Biomedical Optics
Medical Imaging
Spectroscopy

Background:

Quantifying chromophore concentrations in biological tissues using reflectance is challenging.
Conventional Spectroscopic Optical Coherence Tomography (SOCT) methods are limited to specular reflections, often absent in scattering tissues.

Purpose of the Study:

To develop a quantitative method for determining hemoglobin concentrations in highly scattering biological tissues.
To enable simultaneous mapping of oxygen saturation and total hemoglobin concentration, or individual oxyhemoglobin and deoxyhemoglobin concentrations.

Main Methods:

Fitting the dynamic scattering signal spectrum in OCT angiograms using a light propagation forward model.
Quantitative determination of hemoglobin concentrations directly from OCT data.
Ex vivo validation with blood samples at varying pO2 and hematocrit levels.

Main Results:

Successfully quantified hemoglobin concentrations in dynamic scattering signals.
Enabled simultaneous mapping of oxygen saturation and total hemoglobin concentration.
Demonstrated imaging of hemoglobin concentrations in rodent brain and retina.

Conclusions:

The developed SOCT analysis method overcomes limitations of conventional approaches for quantitative chromophore analysis.
This methodology offers potential for clinical applications in assessing tissue oxygenation and hemoglobin levels.
Further discussion addresses confounds like noise and scattering, and future clinical uses.