Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A high-fidelity microfluidic platform reveals retrograde propagation as the main mechanism of α-Synuclein spread in human neurons.

NPJ Parkinson's disease·2025
Same author

Functional, patient-derived 3D tri-culture models of the uterine wall in a microfluidic array.

Human reproduction (Oxford, England)·2024
Same author

Overcoming BCR::ABL1 dependent and independent survival mechanisms in chronic myeloid leukaemia using a multi-kinase targeting approach.

Cell communication and signaling : CCS·2023
Same author

Microfluidic Protocols for the Assessment of Anticancer Therapies in 3D Tumor-Stromal Cocultures.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Calibration of insulin pumps based on discrete doses at given cycle times.

Biomedizinische Technik. Biomedical engineering·2022
Same author

Author Correction: Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Nature metabolism·2022

Related Experiment Video

Updated: Apr 14, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
10:11

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

4.3K

Droplet-interface-bilayer assays in microfluidic passive networks.

Bárbara Schlicht1, Michele Zagnoni1

  • 1Centre for Microsystems and Photonics, Electronic and Electrical Engineering, University of Strathclyde, Glasgow, G1 1XW, UK.

Scientific Reports
|April 25, 2015
PubMed
Summary

This study introduces an automated microfluidic system for creating artificial cell membranes. This technology enables scalable, in vitro studies of cell membrane processes and molecule transport for synthetic biology and drug discovery.

More Related Videos

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
07:57

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Published on: November 10, 2014

8.4K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.7K

Related Experiment Videos

Last Updated: Apr 14, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
10:11

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

4.3K
Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
07:57

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Published on: November 10, 2014

8.4K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.7K

Area of Science:

  • Biophysics
  • Synthetic Biology
  • Microfluidics

Background:

  • Mimicking intracellular and extracellular environments is crucial for studying cell membrane functions.
  • Automated in vitro systems can advance synthetic biology and compound screening.

Purpose of the Study:

  • To develop a microfluidic system for automated artificial lipid bilayer formation.
  • To investigate the scalability and robustness of droplet-interface-bilayer (DIB) techniques.
  • To demonstrate the utility of the system for studying molecular and ionic transport.

Main Methods:

  • Utilized a microfluidic platform for controlled positioning and stabilization of water-in-oil droplets.
  • Employed droplet-interface-bilayer (DIB) techniques for artificial membrane construction.
  • Optimized system parameters including lipid concentration and flow velocities.

Main Results:

  • Successfully developed a scalable and automated system for generating arrays of DIBs.
  • Identified key parameters influencing system performance and robustness.
  • Provided proof-of-concept data for studying diffusive transport across artificial membranes using fluorescence assays.

Conclusions:

  • The integrated microfluidic system offers a robust and scalable platform for artificial cell membrane research.
  • This technology facilitates in vitro investigation of cell membrane biophysics and pharmacology.
  • Enables quantitative analysis of transport phenomena relevant to synthetic biology and drug screening.