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Plasmon waveguide resonance for sensing glycan-lectin interactions.

Isabel Alves1, Ievgen Kurylo2, Yannick Coffinier3

  • 1CBMN, UMR 5248 CNRS, Université de Bordeaux, Bat B14, allée Geoffroy St. Hilaire, Pessac, France.

Analytica Chimica Acta
|April 26, 2015
PubMed
Summary

This study introduces a novel analytical platform for detecting carbohydrate-lectin interactions. Using plasmon waveguide resonance, it achieves sensitive and selective screening with a subnanomolar limit of detection.

Keywords:
Glycan chipPlanar optical waveguidePlasmon waveguide resonance“Click” chemistry

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Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Carbohydrate-modified interfaces are crucial for studying protein-glycan recognition.
  • Label-free plasmonic techniques offer attractive analytical methods.
  • Understanding carbohydrate-lectin interactions is vital in biomedical research.

Purpose of the Study:

  • To develop a sensitive and selective analytical platform for screening carbohydrate-lectin interactions.
  • To utilize plasmon waveguide resonance for label-free detection.
  • To optimize surface architecture for enhanced interaction analysis.

Main Methods:

  • Derivatization of planar optical waveguides (POW) with mannose or lactose moieties.
  • Immobilization of glycans via Cu(I)-catalyzed "click" chemistry.
  • Assessment of lectin binding by monitoring changes in plasmonic response.

Main Results:

  • A novel carbohydrate-lectin interaction screening platform was established.
  • Surface architecture optimization using an oligo(ethylene glycol) spacer enhanced selectivity.
  • A limit of detection (LOD) in the subnanomolar range (0.5 nM) was achieved.

Conclusions:

  • The developed platform offers sensitive and selective screening of carbohydrate-lectin interactions.
  • This method shows significant promise for pharmaceutical and biomedical applications.
  • Further development could lead to advanced diagnostic and research tools.