Production, optimization and characterization of fibrinolytic enzyme by Bacillus subtilis RJAS19

Area of Science:

• Biotechnology
• Enzymology
• Microbiology

Background:

• Nattokinase, a fibrinolytic enzyme, is derived from Bacillus subtilis found in natto.
• Fibrinolytic enzymes are crucial for dissolving blood clots and have therapeutic potential.

Purpose of the Study:

• To produce, purify, and characterize the fibrinolytic enzyme nattokinase.
• To optimize conditions for enhanced enzyme production by Bacillus subtilis.
• To evaluate the enzyme's activity and stability for potential medicinal applications.

Main Methods:

• Isolation and identification of Bacillus subtilis using 16S rDNA sequence analysis.
• Optimization of fermentation conditions (incubation time, temperature, pH, media components).
• Purification of the enzyme using SDS-PAGE and characterization of its activity and stability.

Main Results:

• Bacillus subtilis RJAS 19 was identified as the source of the fibrinolytic enzyme.
• Optimal enzyme production was achieved at 72 hours, 50°C, and pH 9, with lactose and peptone enhancing yield.
• Purified nattokinase exhibited specific activity and stability profiles under varying conditions.

Conclusions:

• The study successfully produced and characterized a potent fibrinolytic enzyme from Bacillus subtilis.
• Optimized production conditions significantly increased enzyme yield.
• The characterized nattokinase shows promise for development as a therapeutic agent in preventive medicine.