A novel method for screening of potential probiotics for high adhesion capability

Area of Science:

• Microbiology
• Probiotics Research
• Gastroenterology

Background:

• Probiotics require robust adhesion to host cells for efficacy.
• Screening methods are needed to identify probiotics with superior adhesion capabilities.
• Gut microbiota modulation is crucial for digestive health.

Purpose of the Study:

• To screen for and identify potential probiotic strains with high adhesion capability.
• To evaluate the in vitro and in vivo adhesion and inhibitory effects of isolated strains.
• To assess the impact of candidate probiotics on gut microbiota composition.

Main Methods:

• Utilized a chemostat model with cultured human feces for isolation.
• Employed denaturing gradient gel electrophoresis (DGGE) for microbial identification.
• Conducted in vitro adhesion assays using HT-29 cells and in vivo animal experiments.

Main Results:

• Isolated Lactobacillus plantarum, Lactobacillus reuteri, and Enterococcus faecalis based on growth and resilience.
• Demonstrated significant adhesion of these strains to HT-29 cells.
• Showed inhibition of pathogenic bacteria (Shigella dysenteriae, Staphylococcus aureus, Enterobacter sakazakii, Escherichia coli) adhesion to HT-29 cells.
• Confirmed in vivo adhesion of Lactobacillus plantarum in mouse intestines, altering gut bacterial populations.

Conclusions:

• Lactobacillus plantarum, Lactobacillus reuteri, and Enterococcus faecalis exhibit promising probiotic properties, including strong adhesion.
• These probiotics can effectively reduce pathogen adhesion to intestinal cells.
• Lactobacillus plantarum demonstrates beneficial effects on gut microbiota composition in vivo.