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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Transposons01:24

Transposons

3.1K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
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Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

21.2K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
14.1K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.4K
LTR Retrotransposons03:08

LTR Retrotransposons

20.6K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
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Related Experiment Video

Updated: Apr 14, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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Transposon insertion mapping with PIMMS - Pragmatic Insertional Mutation Mapping System.

Adam M Blanchard1, James A Leigh1, Sharon A Egan1

  • 1School of Veterinary Medicine and Science, University of Nottingham Loughborough, UK.

Frontiers in Genetics
|April 28, 2015
PubMed
Summary
This summary is machine-generated.

The Pragmatic Insertional Mutation Mapping System (PIMMS) pipeline aids bacterial genome discovery. It identifies conditionally essential genes through streamlined analysis of insertion data, prioritizing further investigation.

Keywords:
INseqTnSeqTraDISsequencingtransposon mapping

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Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma
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Area of Science:

  • Bacteriology
  • Genomics
  • Bioinformatics

Background:

  • The Pragmatic Insertional Mutation Mapping System (PIMMS) pipeline is available for bacterial genome discovery.
  • This system facilitates the identification of conditionally essential genes.

Purpose of the Study:

  • To introduce the PIMMS pipeline for simple conditionally essential genome discovery experiments in bacteria.
  • To enable streamlined analysis of raw sequence data for identifying essential genes.

Main Methods:

  • PIMMS utilizes raw sequence data, a reference genome FASTA file, and a GFF file.
  • The pipeline generates a tabulated output of coding sequences with mapped insertions.
  • Normalized results are provided for streamlined data analysis.

Main Results:

  • PIMMS enables a quick assay of bacterial genomes to identify conditionally essential genes.
  • The system prioritizes results for further investigation on a standard desktop computer.

Conclusions:

  • PIMMS offers an efficient method for discovering conditionally essential genes in bacteria.
  • The pipeline simplifies the analysis of insertional mutation data for genomic research.