Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phosphatic metabolites in keratoconus.

J V Greiner1, J H Lass, W J Reinhart

  • 1Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston.

Experimental Eye Research
|November 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ketone production in ultra marathon runners.

The Journal of sports medicine and physical fitness·2007
Same author

The effect of light exercise upon blood flow velocity determined by laser-Doppler flowmetry.

Journal of medical engineering & technology·2004
Same author

Correlation of lipid layer thickness measurements with fluorescein tear film break-up time and Schirmer's test.

Eye (London, England)·2003
Same author

Comparison of fluorescein break-up time measurement reproducibility using standard fluorescein strips versus the Dry Eye Test (DET) method.

Cornea·2001
Same author

An unusual case of fungal keratitis: Metarrhizium anisopliae.

Cornea·2001
Same author

Cranial rhythmic impulse related to the Traube-Hering-Mayer oscillation: comparing laser-Doppler flowmetry and palpation.

The Journal of the American Osteopathic Association·2001
Same journal

Tanshinone IIA Inhibits Choroidal Neovascularization and Restores Outer Blood-Retinal Barrier Function in Vldlr Knockout Mice.

Experimental eye research·2026
Same journal

Understanding the Ocular Accumulation of Mefuparib and its N-dealkylation metabolite: Pharmacokinetics, Melanin Affinity, and Cellular Disposition.

Experimental eye research·2026
Same journal

Mitochondrial Dysfunction and Diabetic Retinopathy: Research Progress from Pathogenic Mechanisms to Therapeutic Targets.

Experimental eye research·2026
Same journal

Middle-wavelength green ambient light attenuates lens-induced myopia progression and is associated with suppression of the Wnt/β-catenin signaling pathway in guinea pigs.

Experimental eye research·2026
Same journal

Experimental Corneal Alkali Burn Models: Methodological Standards, Biological Outcomes, and Translational Gaps.

Experimental eye research·2026
Same journal

Subretinal injection in the USH1CR31* pig model leads to chorioretinal atrophy that limits evaluation of efficacy of an AAV-mediated gene therapy.

Experimental eye research·2026
See all related articles

Keratoconus corneas show altered phosphorus metabolism compared to normal corneas. Specific unidentified metabolites detected using phosphorus-31 nuclear magnetic resonance spectroscopy may serve as biomarkers for keratoconus.

Area of Science:

  • Biochemistry
  • Ophthalmology
  • Metabolomics

Background:

  • Keratoconus is a progressive thinning disorder of the cornea.
  • Metabolic alterations in keratoconus are not fully understood.
  • Phosphorus-31 nuclear magnetic resonance spectroscopy (31P-MRS) can detect metabolic changes.

Purpose of the Study:

  • To investigate differences in phosphatic metabolites between keratoconus and normal corneas.
  • To identify potential metabolic biomarkers for keratoconus.

Main Methods:

  • Corneal buttons from 16 keratoconus patients and 16 age-matched controls were analyzed.
  • Phosphorus-31 nuclear magnetic resonance spectroscopy (31P-MRS) was used after perchloric acid extraction.
  • Spectra were compared to identify differences in metabolite levels.

Related Experiment Videos

Main Results:

  • Keratoconus corneas exhibited significantly higher levels of nucleoside monophosphates and choline phosphate.
  • Lower levels of adenosine diphosphate were observed in keratoconus corneas.
  • Unique unidentified metabolites were detected in keratoconus but not in control corneas.

Conclusions:

  • Significant alterations in phosphatic metabolites suggest altered corneal metabolism in keratoconus.
  • Unidentified metabolites identified by 31P-MRS may uniquely distinguish keratoconus from normal corneas.
  • These findings provide insights into the metabolic basis of keratoconus.