Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Phylum Firmicutes01:27

Bacterial Phylum Firmicutes

1.3K
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
1.3K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

26.9K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
26.9K
Bacterial Signaling01:30

Bacterial Signaling

43.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
43.6K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

917
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
917
DNA Bacteriophages01:26

DNA Bacteriophages

1.5K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.5K
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

541
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
541

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells.

Molecular microbiology·2026
Same author

Active-learning-guided optimization of cell-free systems for genome-wide transcriptomic profiling reveals progressive layers of regulation.

Nature communications·2026
Same author

A Streamlined and High-Osmolarity <i>Bacillus subtilis</i> Cell-Free Platform for Rapid Characterization of Genetic Regulatory Elements.

ACS synthetic biology·2026
Same author

ModuloStat: An Internet of Things' Path to Continuous Cultures in Mini-Bioreactors.

ACS omega·2026
Same author

Remarkable heterogeneity revealed in the genetic architecture of resistance to a key bacterial pathogen in two commercial rainbow trout populations.

BMC genomics·2025
Same author

sRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus.

Nucleic acids research·2025

Related Experiment Video

Updated: Apr 14, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.8K

Tracking the Elusive Function of Bacillus subtilis Hfq.

Tatiana Rochat1, Olivier Delumeau2, Nara Figueroa-Bossi3

  • 1Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, F-91405, Orsay, France; INRA, UR892, Virologie et Immunologie Moléculaires, F-78352, Jouy-en-Josas, France.

Plos One
|April 28, 2015
PubMed
Summary

The RNA-binding protein Hfq in Bacillus subtilis has a limited role in post-transcriptional regulation, primarily affecting stationary phase survival. Its function differs significantly from Hfq in proteobacteria like Salmonella.

More Related Videos

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

21.3K
Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
09:27

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

18.3K

Related Experiment Videos

Last Updated: Apr 14, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.8K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

21.3K
Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
09:27

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

18.3K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Physiology

Background:

  • The RNA-binding protein Hfq is crucial for adaptive responses in proteobacteria, regulating genes via small non-coding RNAs.
  • The function of Hfq in Gram-positive bacteria, particularly Bacillus subtilis, remains poorly understood and debated.

Purpose of the Study:

  • To investigate the role of Hfq in the Gram-positive bacterium Bacillus subtilis.
  • To compare the growth phenotypes and transcription profiles of wild-type and hfq deletion mutant strains of B. subtilis.

Main Methods:

  • Comparative growth phenotype analysis under diverse conditions.
  • Transcriptomic profiling of wild-type and hfq mutant strains.
  • Functional complementation assays in Salmonella enterica.

Main Results:

  • B. subtilis Hfq deletion mutants showed no significant growth defects under various conditions, except for reduced survival in stationary phase.
  • Transcriptomic analysis revealed approximately one hundred differentially expressed genes, including sporulation-related functions.
  • B. subtilis Hfq partially complemented Salmonella hfq mutants but did not restore all Hfq-dependent regulatory functions.

Conclusions:

  • B. subtilis Hfq plays a specialized role, likely linked to stationary phase physiology, rather than central post-transcriptional regulation.
  • Functional overlap between Hfq proteins from B. subtilis and Salmonella is limited.
  • The conserved nature of Hfq in B. subtilis suggests a specialized but important function in this species.