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Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange
Shaodong Jia1, Lisette Marjavaara, Robert Buckland
1Department of Medical Biochemistry and Biophysics, Umeå University, KBC-Building, 6th Floor, Umeå, 901 87, Sweden.
Abstract:
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.
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